Research related to Plant Breeding

LegumePlus will generate new knowledge for breeding Sainfoin & Birdsfoot Trefoil cultivars. Research has highlighted differences in traits exhibited by various cultivars. The project will identify useful cultivars using DNA markers and gene sequencing to locate beneficial traits. Sowing and co-planting methods will be studied to develop agronomic practices for plant establishment and N fixation.

Scientific Articles/Findings

Poster Presentations

Current Research on the Potential of Bioactive Legumes for Animal Nutrition and Sustainability

I. Mueller-Harvey - University of Reading

Development of molecular tools for targeted improvement of Sainfoin

Agroscope ART - Katharina Kempf, Evelyne Thomet, Roland Kölliker.

First de-novo transcriptome assembly for gene identification, analysis and annotation, transcriptional level study, and molecular marker discovery in Onobrychis viciifolia

NIAB & IBERS - Marina Mora-Ortiz, Lydia Smith, Tom Wood, Leif Skot

Polymorphism and EST-SSR cross-amplification study on a germplasm collection of Onobrychis viciifolia

NIAB & IBERS - Marina Mora-Ortiz, Tom Wood, Leif Skot, Lydia Smith

Abstracts of Published Papers

Benefits of condensed tannins in forage legumes fed to ruminants: importance of structure, concentration and diet composition.

Prof. Irene Mueller-Harvey et al - University of Reading

Published Papers

Benefits of Condensed Tannins in Forage Legumes Fed to Ruminants: Importance of Structure, Concentration, and Diet Composition

Irene Mueller-Harvey, Giuseppe Bee, Frigga Dohme-Meier, Hervé Hoste, Maarit Karonen, Roland Kölliker, Andreas Lüscher, Vincent Niderkorn, Wilbert F. Pellikaan, Juha-Pekka Salminen, Leif Skøt, Lydia M.J. Smith, Stig M. Thamsborg, Paul Totterdell, Ian Wilkinson, Andrew R. Williams, Blasius N. Azuhnwi, Nicolas Baert, Anja Grosse Brinkhaus, Giuseppe Copani, Olivier Desrues.

Characterization of novel SSR markers in diverse sainfoin (Onobrychis viciifolia) germplasm

Katharina Kempf, Marina Mora-Ortiz, Lydia M. J. Smith, Roland Kölliker, Leif Skøt

De-novo transcriptome assembly for geneidentification analysis annotation and molecular marker discovery in Onobrychis viciifolia

Marina Mora-Ortiz, Martin T. Swain, Martin J. Vickers, Matthew J. Hegarty, Rhys Kelly, Lydia M. J. Smith1and Leif Skøt

Drought effects on proanthocyanidins in sainfoin (Onobrychis viciifolia) are dependent on the plant’s ontogenetic stage.

Malisch CS, Salminen J-P, Kölliker R, Engström MT, Suter D, Studer B, Lüscher A (2016)

Evidence and consequences of self-fertilisation in the predominantly outbreeding forage legume Onobrychis viciifolia.

Kempf K, Grieder C, Walter A, Widmer F, Reinhard S, Kölliker R (2015)

Improving performance and proanthocyandidins of the forage legume sainfoin (Onobrychis viciifolia Scop.) Euphytica

Kölliker R, Kempf K, Malisch CS, Lüscher A (2017)

Large variability of proanthocyanidin content and composition in sainfoin (Onobrychis viciifolia).

Malisch CS, Lüscher A, Baert N, Engström MT, Studer B, Fryganas C, Mueller-Harvey I, Salminen J-P (2015)

Marker-trait association analysis for agronomic and compositional traits in sainfoin (Onobrychis viciifolia).

Kempf K, Malisch CS, Grieder C, Widmer F, Kölliker R (2017)

Multifunctional benefits of sainfoin mixtures: Effects of partner species, sowing density and cutting regime.

Malisch CS, Suter D, Studer B, Lüscher A (2017)

Potential of legume-based grassland-livestock systems in Europe: a review.

Lüscher A, Mueller-Harvey I, Soussana JF, Rees RM, Peyraud JL (2014)

Results from The Healthy Hay Project

Die Erhaltung der Esparsette (Onobrychis viciifolia) Schlussbericht 2007-2010 (Conservation of sainfoin (Onobrychis viciifolia) genetic resources, final reportr 2007-2010)

ART, Switzerland - Beat Boller and Eric Schweizer AG, Project NAP (Swiss National Action Plan for the Preservation and Sustainable Use of Plant Genetic Resources)

Die Erhaltung der Esparsette (Onobrychis viciifolia) Zwischenbericht 2007 (Conservation of sainfoin (Onobrychis viciifolia) genetic resources, intermediate report for 2007)

ART, Switzerland - Beat Boller and Eric Schweizer AG, Project NAP (Swiss National Action Plan for the Preservation and Sustainable Use of Plant Genetic Resources)

Die Erhaltung der Esparsette (Onobrychis viciifolia) Zwischenbericht 2008 Conservation of sainfoin (Onobrychis viciifolia) genetic resources, intermediate report for 2008)

ART, Switzerland - Beat Boller and Eric Schweizer AG, Project NAP (Swiss National Action Plan for the Preservation and Sustainable Use of Plant Genetic Resources)

Die Erhaltung der Esparsette (Onobrychis viciifolia) Zwischenbericht 2009 (Conservation of sainfoin (Onobrychis viciifolia) genetic resources, intermediate report for 2009)

ART, Switzerland - Beat Boller and Eric Schweizer AG, Project NAP (Swiss National Action Plan for the Preservation and Sustainable Use of Plant Genetic Resources)

Establishment and production of common sainfoin (Onobrychis viciifolia Scop.) in the UK. 1. Effects of sowing date and autumn management on establishment and yield

Z. Liu, G. P. F. Lane and W. P. Davies The Royal Agricultural College, Cirencester

EST-SSR cross-amplification and genetic similarity in Onobrychis genus. Genet Resour Crop Evol 2012. 59, 253-260

Ignacio Delgado, Jose Valderrabano, Ana Wunsch.

Genetic characterisation of a collection of sainfoin

CITA

Molecular Markers for Phylogenetics of Onobrychis Species

NIAB - Christine Hayot, Ilya Gadjev, Lydia Smith and Terry Brown

Morphology, tannin concentration and forage value of 15 Swiss accessions of sainfoin (Onobrychis viciifolia Scop.) as influenced by harvest time and cultivation site. Grass Forage Sci.in press.

ALP and ART, Switzerland - Azuhnwi, B. N., B. Boller, M. Martens, F. Dohme-Meier, S. Ampuero, S. Günter, M. Kreuzer, and H. D. Hess. 2011.

Near Infrared Spectroscopy (NIRS) for Screening Sainfoin Germplasm

Reading University - Martin-Lorenzo M, Hayot C, Stringano E, Falco-Sales L, Smith L, Barnes R, Oliver J, Brown R H, Mueller-Harvey I

PhD Thesis: SAINFOIN (ONOBRYCHIS VICIIFOLIA), A FORAGE LEGUME WITH GREAT POTENTIAL FOR SUSTAINABLE AGRICULTURE, AN INSIGHT ON ITS MORPHOLOGICAL, AGRONOMICAL, CYTOLOGICAL AND GENETIC CHARACTERISATION

NIAB - Christine Hayot

Phylogenetic characterisation of Onobrychis species with special focus on the forage crop Onobrychis viciifolia Scop. Genet Resour Crop Evol 2012, 59, 1777–1788.

Christine Hayot Carbonero, Franck Carbonero, Lydia M. J. Smith,Terence A. Brown

Pictures demonstrating some typical varietal differences between Sainfoin accessions

CITA

Pre-Breeding and Development of Sainfoin (Onobychis vicifolia)

NIAB - Christine Hayot, Ilya Gadjev, Guillaume Barbier and Lydia MJ Smith

Sainfoin (Onobrychis viciifolia): a beneficial forage legume. Plant Genet. Res.: Util. Charact. 2011, 9, 70-85.

Hayot Carbonero, C., Mueller-Harvey, I., Brown, T.A, Smith, L.